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Status
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Public on Apr 17, 2019
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Title
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mRNAseq in control (WT) and Nes:Cre_Qk cKO
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Organism
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Mus musculus
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Experiment type
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Expression profiling by high throughput sequencing
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Summary
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We sequenced mRNA from mouse E14.5 embryonic cortex to compare gene expression level and alternative splicing events between 2 control (WT) and 2 Qk cKO.
A set of tissue specific splicing factors are thought to govern alternative splicing events during neural progenitors (NPC) to neuron transition by regulating neuron specific exons. Here we proposed one such a factor, RNA-binding protein Qki5, which is specifically expressed in neural stem cells. We performed mRNAseq analysis by using mRNAs obtained from developing cerebral cortices in Qk conditional knockout (cKO) mice. Expectedly, we found huge numbers of alternative splicing changes between control and conditional knock-out relative to that of transcripts level changes. Furthermore, DAVID and Meta-scape analysis revealed that affected spliced genes are involved in axon-development and microtubule-based process. Among these, Ninein protein coding mRNA is listed as a Qk protein dependent alternative splicing targets. Interestingly, this exon encodes very long poly-peptides (2,121 nt) and is known as a previously defined dynamic RNA switch during NPC-to-neuron transition. In addition, we validated that the regulation of this large exon is consistent with Qki5 dependent alternative exon inclusion mode obtained from our previous Qki5 HITS-CLIP analysis. Together Qki5 will add to a list factor of alternative splicing in NPC-to-neuron transition.
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Overall design
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mRNA profiles obtained from 2 different control(WT) and 2 Qk cKO were generated by deep sequencing using Illumina NextSeq500.
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Contributor(s)
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Hayakawa-Yano Y
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Yano M
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Citation(s)
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30813567
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35942699
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Submission date
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Dec 17, 2018
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Last update date
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Aug 24, 2022
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Contact name
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Masato Yano
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E-mail(s)
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myano@med.niigata-u.ac.jp
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Organization name
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Niigata University
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Department
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Division of Neurobiology and Anatomy
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Street address
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757, Ichibancho, Asahimachidori, Chuo-ku, Niigata-shi,
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City
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Niigata
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ZIP/Postal code
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950-0907
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Country
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Japan
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Platforms (1)
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GPL21103
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Illumina HiSeq 4000 (Mus musculus)
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Samples (4)
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Relations
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BioProject
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PRJNA510335
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SRA
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SRP173594
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Supplementary file
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Size
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Download
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File type/resource
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GSE123927_RAW.tar
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7.3 Mb
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(http)
(custom)
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TAR (of TXT)
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SRA Run Selector
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Raw data are available in SRA
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Processed data provided as supplementary file
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