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Status
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Public on Jun 12, 2019
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Title
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Effect of BRD4 inhibition in leukemic stem cells (Microarray)
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Organism
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Mus musculus
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Experiment type
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Expression profiling by array
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Summary
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DNA Methyltransferase 3A (DNMT3A) is frequently mutated in various hematopoietic malignancies. We report that DNMT3A mutational ‘hotspot’ at Arg882 (i.e., DNMT3A-R882H) cooperates with constitutively activated RAS in transforming murine hematopoietic stem/progenitor cells (HSPCs) ex vivo and inducing acute leukemias in vivo. Mechanistically, DNMT3A-R882H induced DNA hypomethylation facilitates gene enhancer/promoter activation.
In this dataset, we provided microarray data showing effect of BRD4 inhibitor I-BET151 on gene expression in leukemic stem cells transformed by DNMT3A R882H and NRAS G12D.
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Overall design
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Gene expression data were generated using RH-RAS leukemia stem cell lines (transformed by DNMT3A R882H and NRAS G12D) treated with 100 nM I-BET-151 (a Brd4-specific small-molecule inhibitor) for 24 hours.
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Contributor(s)
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Lu R
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Wang GG
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Citation(s)
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31164355
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Submission date
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May 02, 2019
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Last update date
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Jun 14, 2019
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Contact name
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Rui Lu
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E-mail(s)
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ruilu1@uabmc.edu
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Organization name
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Univ of Alabama at Birmingham
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Street address
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1824 6th Ave S
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City
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Birmingham
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State/province
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AL
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ZIP/Postal code
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35294
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Country
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USA
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Platforms (1)
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GPL17400
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[MoGene-2_1-st] Affymetrix Mouse Gene 2.1 ST Array [transcript (gene) version]
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Samples (4)
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GSM3745986
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RH-RAS leukemia stem cell line, DMSO_24hr_1
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GSM3745987
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RH-RAS leukemia stem cell line, DMSO_24hr_2
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GSM3745988
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RH-RAS leukemia stem cell line, IBET151_24hr_1
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GSM3745989
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RH-RAS leukemia stem cell line, IBET151_24hr_2
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Relations
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BioProject
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PRJNA540839
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